LOCUS       sequence-152325-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  high FRET control for ActTS-GR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Yamashita S, Tsuboi T, Ishinabe N, Kitaguchi T, Michiue T
  TITLE     Wide and high resolution tension measurement using FRET in embryo.
  JOURNAL   Sci Rep. 2016 Jun 23;6:28535. doi: 10.1038/srep28535.
  PUBMED    27335157
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-06-23"; volume: "6:28535. doi"; pages: " 
            10.1038/srep28535"
COMMENT     Sequence Label: hiActTS-GR
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(203..224)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     552..571
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     811..832
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      121 atccggtgga tcccgggccc gcggtacctt agcggccgct cttgtacagc tcgtccatgc
      181 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      241 tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
      301 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
      361 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
      421 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
      481 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
      541 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
      601 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
      661 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
      721 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
      781 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
      841 ccaccccggt ga
//
