LOCUS       sequence-152327-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB060c
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(459..482)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atcaatattg ttgatgataa tattaagaag taccacacgg aatataccta aacggagcca
       61 atgtacgcaa aaaaccccgc ttcagcgggg ttttttcgcc aaaaaaaacc ccgccctgtc
      121 aggggcgggg ttttttttta gggccctact gactgttagc gtttcggcgc cggataatgc
      181 agcgtggtga tcagaccatc cgcaaccgtg ctatggtgat gcaccagttc tgccagggta
      241 ttgaagcgag attcactaga aacgtacagt ttgccatcgc tggcggtgtt gatacggtag
      301 tgatacacgc gaccttcata acgcagagaa atgctgcgct ggcccgggct actttcactt
      361 tcacgaacca gaaaagaacc attaatgcca gagctcagca ggtattcggc cgcgttacgg
      421 ctcacagggc catgatacca gctgtgtttt tccagactct tgtcatcgtc gtccttgtag
      481 tccccaaacg tctcttcagg ccactgacta gcgataactt tccccacaac ggaacaactc
      541 tcattgcatg ggatcattgg gtactgtggg tttagtggtt gtaaaaacac ctgaccgcta
      601 tccctgatca gtttcttgaa ggtaaactca tcacccccaa gtctggctat gcagaaatca
      661 cctggctcaa cagcctgctc agggtcaacg agaattaaca ttccgtcagg aa
//
