LOCUS       sequence-152329-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB076i3
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(454..477)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tattgttgat gataatatta agaagtacca cacggaatat acctaaacgg agccaatgta
       61 cgcaaaaaac cccgcttcag cggggttttt tcgccaaaaa aaaccccgcc ctgtcagggg
      121 cggggttttt ttttagggcc ctactgactg ttagcgtttc ggcgccggat aatgcagcgt
      181 ggtgatcaga ccatccgcaa ccgtgctatg gtgatgcacc agttctgcca gggtattgaa
      241 gcgagattca ctagaaacgt acagtttgcc atcgctggcg gtgttgatac ggtagtgata
      301 cacgcgacct tcataacgca gagaaatgct gcgctggccc gggctacttt cactttcacg
      361 aaccagaaaa gaaccattaa tgccagagct cagcaggtat tcggccgcgt tacggctcac
      421 agggccatga taccagctgt gtttttccag actcttgtca tcgtcgtcct tgtagtctac
      481 gaattttacc ctcgcttcca cgacaacacc gataatcttg cagtttccgt tgataggagt
      541 cataggccat gaaggattca ggcctttcag gtacttctga ccgccatcta tgaccagttt
      601 cttgaatgtt gcttcgttcg cgtcagtcag tttggctaca acaaggcttc cattcactgg
      661 ctcccgtcca gtatctacta acaccatatg accttcaggg atgctttgac ctacaggtga
      721 ggtcatggaa tcaccttcaa ccttcagcca gaatccattg cctaataagt taacgtcact
      781 gtcataccat tcatcaatgt ccttgatatc gtagg
//
