LOCUS       sequence-152333-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB078d5
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(155..178)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atattgttga tgataatatt aagaagtacc acacggaata tacctaaacg gagccaatgt
       61 acgcaaaaaa ccccgcttca gcggggtttt ttcgccaaaa aaaaccccgc cctgtcaggg
      121 gcggggtttt tttttagggc cctactgact gttacttgtc atcgtcgtcc ttgtagtcta
      181 cgaattttac cctcgcttcc acgacaacac cgataatctt gcagtttccg ttgataggag
      241 tcataggcca tgaaggattc aggcctttca ggtacttctg accgccatct atgaccagtt
      301 tcttgaatgt tgcttcgttc gcgtcagtca gtttggctac aacaaggctt ccattcactg
      361 gctcccgtcc agtatctact aacaccatat gaccttcagg gatgctttga cctacaggtg
      421 aggtcatgga atcaccttca accttcagcc agaatccatt gcctaataag ttaacgtcac
      481 tgtcatacca ttcatcaatg tccttgatat cgtagggttc acaagcttca caccacgaac
      541 gagctctaac catgctaatc aatggatatt tccctttggg ctcaacgtgc ccaacaaatc
      601 taacattcga atcagaggtg ccattgagca gccagtcaac acttacgcca agagctgacg
      661 caagttctgg taaaaagcgt ggtcgcttag ttttaccgtt ttcgagctgc tctatagact
      721 gctgggtagt ccccaccttt tgagcaagtt cagcctggtt aagtccaagc tgaattcttt
      781 tgctttttac cctgctgctg atactcattt ttttttcctc
//
