LOCUS       sequence-152343-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic Cre-dependent expression of TRPV4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, 
            Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann 
            CD, Guler AD
  TITLE     Genetically targeted magnetic control of the nervous system.
  JOURNAL   Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
  PUBMED    26950006
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265."
COMMENT     Sequence Label: pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(71..104)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(155..188)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(236..259)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(854..877)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attctagagt
       61 cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg
      121 attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac
      181 gaagttatgg cgcgccggat ccttagctag ccactcggga ggagcctcca ccccctttgt
      241 catcgtcgtc tttatagtcg ctcccgccac ccccgcgtgc gctttcatta tcactgtctc
      301 ccagggtgtg cttgtcaaag agatattccg ccaagccaga ttcgggcgct cccatcttgc
      361 gcaagttggt cacgtggtca cccaattctt tgatggcttt cacctgctca ttcaggtaat
      421 gtgtctcaat gaagtcacac aaatgggggt catttttgtc agtggccagt ttgtgcagtt
      481 ccagtagtga ctgattcaca tttttttcca aatgtaatgc acactccatt gcattcagcc
      541 cgctctccca gtcatcacag tctggtttct tgatatcctg aaggaagatt cggccacctc
      601 gttggttctg cagcttcatc agtttctcag catgttccct ctcctcatga gattggtgaa
      661 gaaagtattt ggcaaagttc ttcaaagcca catcatcgcg gtcaaagtag taagacatgg
      721 acaggtaaac gtaggaggcg tagagctcca ggttgatctg gcggttgatg gcggcctctg
      781 agtcctggtg gtagttctgg cgcacctgcg aggtggacgc ggtcgtcatg acgcgggagg
      841 agccgccgcc gcctttatca tcatcatctt tataatcgga gccgccgccg ccgcgggcgt
      901 cgtgcttgag agtgagcctt tcgaagaga
//
