LOCUS       sequence-152345-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses APEX in the mitochondrial intermembrane space..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Hung V, Zou P, Rhee HW, Udeshi ND, Cracan V, Svinkina T, Carr SA, 
            Mootha VK, Ting AY
  TITLE     Proteomic mapping of the human mitochondrial intermembrane space in 
            live cells via ratiometric APEX tagging.
  JOURNAL   Mol Cell. 2014 Jul 17;55(2):332-41. doi: 
            10.1016/j.molcel.2014.06.003. Epub 2014 Jul 4.
  PUBMED    25002142
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.06.003"; journalName: "Mol Cell"; date: 
            "2014-07-17- 17"; volume: "55"; issue: "2"; pages: "332-41"
COMMENT     Sequence Label: IMS-APEX2
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             268..309
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctgtcgggat caacaagttt gtacaaaaaa gcaggcttcg aaggagatag aaccatgtac
       61 cggctcctgt caagcgtgac agctcgggct gcggccaccg caggcccagc ctgggacgga
      121 gggcggcgcg gggcgcacag gcgaccgggc ctgcctgtgc tgggccttgg ttgggccggc
      181 ggcctggggc tcgggctggg gctggctctc ggcgcgaagc tggtggtcgg gctgcggggc
      241 gccgtcccca ttcagtccag ggatccaggc aagcccatcc ccaaccccct gctgggcctg
      301 gacagcaccg gaaagtctta cccaactgtg agtgctgatt accaggacgc cgttgagaag
      361 gcgaagaaga agctcagagg cttcatcgct gagaagagat gcgctcctct aatgctccgt
      421 ttggcattcc actctgctgg aacctttgac aagggcacga agaccggtgg acccttcgga
      481 accatcaagc accctgccga actggctcac agcgctaaca acggtcttga catcgctgtt
      541 aggcttttgg agccactcaa ggcggagttc cctattttga gctacgccga tttctaccag
      601 ttggctggcg ttgttgccgt tgaggtcacg ggtggaccta aggttccatt ccaccctgga
      661 agagaggaca agcctgagcc accaccagag ggtcgcttgc ccgatcccac taagggttct
      721 gaccatttga gagatgtgtt tggcaaagct atggggctta ctgacca
//
