LOCUS       sequence-152347-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB085e
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(471..494)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gataatatta agaagtacca cacggaatat acctaaacgg agccaatgta cgcaaaaaac
       61 cccgcttcag cggggttttt tcgccaaaaa aaaccccgcc ctgccagggg cggggttttt
      121 ttttagggcc ctactgactg ttagttgaga tgagtgatca gatagatctt cacgtcagta
      181 cgggcttctg caccacgggt gtctgtagcc acgacatcga actcgaacat gccgaccata
      241 gtggcgctcg ggttgaagtt gagggacagg acgccgctgc tcgggtgcag cgtgaaggcg
      301 gactcacgca cgccctccag cgaggggtcc accaccatcg tgtccaggtc catcgtgtac
      361 gttatgtcta ggccttcggt atgtgtcgca tgaacagtga gtagttccct gttggatgta
      421 tccgtctcgt agatgccggc ggtgtagatc tcacgctcga atactggccg cttgtcatcg
      481 tcgtccttgt agtctacgaa ttttaccctc gcttccacga caacaccgat aatcttgcag
      541 tttccgttga taggagtcat aggccatgaa ggattcaggc ctttcaggta cttctgaccg
      601 ccatctatga ccagtttctt gaatgttgct tcgttcgcgt cagtcagttt ggctacaaca
      661 aggcttccat tcactggctc ccg
//
