LOCUS       sequence-152350-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB049b
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    383..404
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(419..424,425..442,443..449)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    457..473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc
       61 tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct
      121 gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta
      181 agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat
      241 acgggttact gatgatgaac aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
      301 aagactgggc ctttcgtttt gctgaggaga cttagggacc ctggtttccc gactggaaag
      361 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      421 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      481 cacctgcagg tgcagtaagg aggaaaaaaa aatgggcagc tctgtgagta gcgttccgac
      541 caaactggaa gtggttgcag caaccccgac gagcctgctg atttcttggg atgccccgat
      601 gtctagtagc tctgtgtatt actatcgtat cacctacggt gaaacgggcg gtaacagccc
      661 ggtgcaggaa tttacggttc
//
