LOCUS       sequence-152351-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB082h
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             507..779
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     CDS             789..818
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
       61 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
      121 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
      181 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
      241 atacgggtta ctgatgatga acaagaggac atccggtcaa ataaaacgaa aggctcagtc
      301 gaaagactgg gcctttcgtt ttgctgagga ttctagagca cagctaacac cacgtcgtcc
      361 ctatctgctg ccctaggtct atgagtggtt gctggataac tttacgggca tgcataaggc
      421 tcggtatcta tattcaggga gaccacaacg gtttccctct acaaataatt ttgtttaact
      481 tttactagag taaggaggaa aaaaaaatgg cacgcgtaac tgttcaggac gctgtagaga
      541 aaattggtaa ccgttttgac ctggtactgg tcgccgcgcg tcgcgctcgt cagatgcagg
      601 taggcggaaa ggatccgctg gtaccggaag aaaacgataa aaccactgta atcgcgctgc
      661 gcgaaatcga agaaggtctg atcaacaacc agatcctcga cgttcgcgaa cgccaggaac
      721 agcaagagca ggaagccgct gaattacaag ccgttaccgc tattgctgaa ggtcgtcgtg
      781 cggccgcgga acaaaagctt atttctgaag aggacttgac aagaga
//
