LOCUS       sequence-152359-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Soybean APEX2 anchored to cytosolic face of outer mitochondrial 
            membrane with C-terminal targeting sequence of last 31aa of human 
            mitochondrial antiviral-signaling protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: APEX2-OMM
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctgtcgggat caacaagttt gtacaaaaaa gcaggcttcg aaggagatag aaccatggac
       61 tacaaggatg acgacgataa gggaaagtct tacccaactg tgagtgctga ttaccaggac
      121 gccgttgaga aggcgaagaa gaagctcaga ggcttcatcg ctgagaagag atgcgctcct
      181 ctaatgctcc gtttggcatt ccactctgct ggaacctttg acaagggcac gaagaccggt
      241 ggacccttcg gaaccatcaa gcaccctgcc gaactggctc acagcgctaa caacggtctt
      301 gacatcgctg ttaggctttt ggagccactc aaggcggagt tccctatttt gagctacgcc
      361 gatttctacc agttggctgg cgttgttgcc gttgaggtca cgggtggacc taaggttcca
      421 ttccaccctg gaagagagga caagcctgag ccaccaccag agggtcgctt gcccgatccc
      481 actaagggtt ctgaccattt gagagatgtg tttggcaaag ctatggggct tactgaccaa
      541 gatatcgttg ctctatctgg gggtcacact attggagctg cacacaagga gcgttctgga
      601 tttgagggtc cctggacctc taatcctctt attttcgaca actcatactt cacggagttg
      661 ttgagtggtg agaaggaagg tctccttcag ctaccttctg acaaggctct tttgtctgac
      721 cctgtattcc gccctctcgt tgacaaatat gcagcggacg aagatgcctt ctttgctgat
      781 tacgctgagg ctcac
//
