LOCUS       sequence-152361-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Soybean APEX2 anchored to cytosolic face of outer mitochondrial 
            membrane with C-terminal targeting sequence of last 31aa of human 
            mitochondrial antiviral-signaling protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: APEX2-OMM
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..72)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcgccaccgg ttagcgctag ctcattacta cgtagaatcg agaccgagga gagggttagg
       61 gataggctta ccaaccactt tgtacaagaa agctgggtcc tagtgcagac gccgccggta
      121 cagcaccacc aggagtgtga ctaccagcac ccctgtcaca gccacctgga gccacagagc
      181 cccaggtgag ggcctgaatt cggcatcagc aaacccaagc tcggaaagct tttggtgagc
      241 ctcagcgtaa tcagcaaaga aggcatcttc gtccgctgca tatttgtcaa cgagagggcg
      301 gaatacaggg tcagacaaaa gagccttgtc agaaggtagc tgaaggagac cttccttctc
      361 accactcaac aactccgtga agtatgagtt gtcgaaaata agaggattag aggtccaggg
      421 accctcaaat ccagaacgct ccttgtgtgc agctccaata gtgtgacccc cagatagagc
      481 aacgatatct tggtcagtaa gccccatagc tttgccaaac acatctctca aatggtcaga
      541 acccttagtg ggatcgggca agcgaccctc tggtggtggc tcaggcttgt cctctcttcc
      601 agggtggaat ggaaccttag gtccacccgt gacctcaacg gcaacaacgc cagccaactg
      661 gtagaaatcg gcgtagctca aaatagggaa ctccgccttg agtggctcca aaagcctaac
      721 agcgatgtca agaccgttgt tagcgctgtg agccagttcg gcagggtgct ttgat
//
