LOCUS       sequence-152365-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 
            1 protein MICU1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: MICU-APEX2
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..49
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctctctggc tactgtcggg atcaacaagt ttgtacaaaa aagcaggctt cgaaggagat
       61 agaaccatgt ttcgtctgaa ctcactttct gctttggcag aactggctgt gggttctcga
      121 tggtaccatg gaggatcaca gcccatccag atccggcgaa gactaatgat ggtggctttc
      181 ctgggagcat ctgcagtaac tgcaagtact ggtcttttgt ggaagagggc ccatgcagaa
      241 tctccaccat gtgtagacaa cctaaaaagt gacatcggtg ataaagggaa gaataaagat
      301 gaaggggatg tttgtaacca tgagaaaaag actgcagatc ttgcccctca cccagaagag
      361 aaaaagaaga aacgttctgg attcagagac agaaaagtga tggaatatga gaataggatt
      421 cgagcctact ccacgccaga caaaatcttc cgatattttg ccaccttgaa agtcatcagt
      481 gagcctggtg aagcagaagt gtttatgaca ccagaagatt ttgtgcgatc cataacaccc
      541 aatgaaaaac aaccagaaca cttgggtctg gatcaatata taataaaacg ctttgatgga
      601 aagaaaattt cccaggaacg agaaaaattt gctgatgaag gcagtatatt ttacaccctt
      661 ggagaatgtg ggctcatatc cttttcagac tacattttcc tcacaactgt tctttccact
      721 cctcagagaa a
//
