LOCUS       sequence-152366-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB092a
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(455..478)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atattgttga tgataatatt aagaagtacc acacggaata tacctaaacg gagccaatgt
       61 acgcaaaaaa ccccgcttca gcggggtttt ttcgccaaaa aaaaccccgc cctgtcaggg
      121 gcggggtttt tttttagggc cctactgact gttagcgttt cggcgccgga taatgcagcg
      181 tggtgatcag accatccgca accgtgctat ggtgatgcac cagttctgcc agggtattga
      241 agcgagattc actagaaacg tacagtttgc catcgctggc ggtgttgata cggtagtgat
      301 acacgcgacc ttcataacgc agagaaatgc tgcgctggcc cgggctactt tcactttcac
      361 gaaccagaaa agaaccatta atgccagagc tcagcaggta ttcggccgcg ttacggctca
      421 cagggccatg ataccagctg tgtttttcca gactcttgtc atcgtcgtcc ttgtagtcta
      481 cgaattttac cctcgcttcc acgacaacac cgataatctt gcagtttccg ttgataggag
      541 tcataggcca tgaaggattc aggcctttca ggtacttctg accgccatct atgaccagtt
      601 tcttgaatgt tgcttcgttc gcgtcagtca gtttggctac aacaaggctt ccattcactg
      661 gctcccgtcc agtatctact aacaccatat gaccttcagg gatgctttga cctacaggtg
      721 aggtcatgga atcaccttca accttcagcc agaatccatt gcctaataag ttaacgtcac
      781 tgtcatacca ttcatcaatg tccttgatat cgtagggttc acaagct
//
