LOCUS       sequence-152367-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 
            1 protein MICU1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: MICU-APEX2
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..70)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gccaccggtt agcgctagct cattactacg tagaatcgag accgaggaga gggttaggga
       61 taggcttacc aaccactttg tacaagaaag ctgggtctta ggcatcagca aacccaagct
      121 cggaaagctt ttggtgagcc tcagcgtaat cagcaaagaa ggcatcttcg tccgctgcat
      181 atttgtcaac gagagggcgg aatacagggt cagacaaaag agccttgtca gaaggtagct
      241 gaaggagacc ttccttctca ccactcaaca actccgtgaa gtatgagttg tcgaaaataa
      301 gaggattaga ggtccaggga ccctcaaatc cagaacgctc cttgtgtgca gctccaatag
      361 tgtgaccccc agatagagca acgatatctt ggtcagtaag ccccatagct ttgccaaaca
      421 catctctcaa atggtcagaa cccttagtgg gatcgggcaa gcgaccctct ggtggtggct
      481 caggcttgtc ctctcttcca gggtggaatg gaaccttagg tccacccgtg acctcaacgg
      541 caacaacgcc agccaactgg tagaaatcgg cgtagctcaa aatagggaac tccgccttga
      601 gtggctccaa aagcctaaca gcgatgtcaa gaccgttgtt agcgctgtga gccagttcgg
      661 cagggtgctt gatggttccg aagggtccac cggtcttcgt gcc
//
