LOCUS       sequence-152374-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a 
            visual screen for Cas9-free plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Gao X, Chen J, Dai X, Zhang D, Zhao Y
  TITLE     An effective strategy for reliably isolating heritable and Cas9-free
            Arabidopsis mutants generated by CRISPR/Cas9-mediated genome 
            editing.
  JOURNAL   Plant Physiol. 2016 May 15. pii: pp.00663.2016.
  PUBMED    27208253
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2016 May 15. pii: pp.00663.2016."
COMMENT     Sequence Label: pHDE-35S-Cas9-mCherry
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggagaggaca cgatatggac aagaagtact ccattgggct cgatatcggc acaaacagcg
       61 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
      121 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
      181 gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
      241 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
      301 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc
      361 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
      421 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
      481 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
      541 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
      601 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
      661 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
      721 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact
      781 tcgacctgg
//
