LOCUS       sequence-152375-         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a 
            visual screen for Cas9-free plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Gao X, Chen J, Dai X, Zhang D, Zhao Y
  TITLE     An effective strategy for reliably isolating heritable and Cas9-free
            Arabidopsis mutants generated by CRISPR/Cas9-mediated genome 
            editing.
  JOURNAL   Plant Physiol. 2016 May 15. pii: pp.00663.2016.
  PUBMED    27208253
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2016 May 15. pii: pp.00663.2016."
COMMENT     Sequence Label: pHDE-35S-Cas9-mCherry
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    504..528
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cttgatgatg gccatgttat ccattgttgg taccgttttg ctatttgtgt atgttttctt
       61 gttttggttt gagtgaagag tgtagtgaag ggttggtttg tatttatagg tgagaaagga
      121 gatttgcatg gcaatcacgt gtaagaatgc atgcatgcat gagtgtgtgt ggagagagag
      181 agatgctctt acgttgttgt cgggattatg tgttggaaaa gagtgacaag tgacgaacaa
      241 aacaatacca ctcatcatag ctccgcttat gcatgctatg tgtgatttgt agctcttgtt
      301 ctgacatgac actatgaaca attttttaaa aagaaggtta aacttttatt taaataaaaa
      361 ccctatgtta atttggtttc aatcgagtaa gatgagaagt caatcttttg atggtagcta
      421 gtgatgttgg aaatatagtt gtgccagctt aggtctcata gttgagacca agcttcactt
      481 cactagttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
      541 agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
      601 tttgtatgtg catgccaacc acagggttcc cctcggg
//
