LOCUS       sequence-152376-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a 
            visual screen for Cas9-free plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Gao X, Chen J, Dai X, Zhang D, Zhao Y
  TITLE     An effective strategy for reliably isolating heritable and Cas9-free
            Arabidopsis mutants generated by CRISPR/Cas9-mediated genome 
            editing.
  JOURNAL   Plant Physiol. 2016 May 15. pii: pp.00663.2016.
  PUBMED    27208253
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2016 May 15. pii: pp.00663.2016."
COMMENT     Sequence Label: pHDE-35S-Cas9-mCherry
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      248..500
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
ORIGIN
        1 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
       61 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      121 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      181 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
      241 gagagctctt ggactcccat gttggcaaag gcaaccaaac aaacaatgaa tgatccgctc
      301 ctgcatatgg ggcggtttga gtatttcaac tgccatttgg gctgaattga agacatgctc
      361 ctgtcagaaa ttccgtgatc ttactcaata ttcagtaatc tcggccaata tcctaaatgt
      421 gcgtggcttt atctgtcttt gtattgtttc atcaattcat gtaacgtttg cttttcttat
      481 gaattttcaa ataaattatc gtattgagac cgaattctgt ctagacctaa ctgagtaagc
      541 tagcttgttc gagtattatg gcattgggaa aactgttttt cttgtaccat ttgttgtgct
      601 tgtaatttac tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg
      661 gagaagagtt aatgaatgat atggtccttt tgttcattct caaattaata ttatttgttt
//
