LOCUS       sequence-152378-         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a 
            visual screen for Cas9-free plants. Enable production of two gRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Gao X, Chen J, Dai X, Zhang D, Zhao Y
  TITLE     An effective strategy for reliably isolating heritable and Cas9-free
            Arabidopsis mutants generated by CRISPR/Cas9-mediated genome 
            editing.
  JOURNAL   Plant Physiol. 2016 May 15. pii: pp.00663.2016.
  PUBMED    27208253
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2016 May 15. pii: pp.00663.2016."
COMMENT     Sequence Label: pHDE-35S-Cas9-mCherry-UBQ
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat
       61 accgatcgcc acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggag
      121 acggccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat
      181 cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc
      241 ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca
      301 atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat
      361 ctgaggaaga agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg
      421 ctggcgcata tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac
      481 aacagcgatg tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa
      541 gagaacccga tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc
      601 aaatcccggc ggctcgaaaa cctcatcgca cagctccctg g
//
