LOCUS       sequence-152380-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a 
            visual screen for Cas9-free plants. Enable production of two gRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Gao X, Chen J, Dai X, Zhang D, Zhao Y
  TITLE     An effective strategy for reliably isolating heritable and Cas9-free
            Arabidopsis mutants generated by CRISPR/Cas9-mediated genome 
            editing.
  JOURNAL   Plant Physiol. 2016 May 15. pii: pp.00663.2016.
  PUBMED    27208253
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2016 May 15. pii: pp.00663.2016."
COMMENT     Sequence Label: pHDE-35S-Cas9-mCherry-UBQ
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    509..533
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gactccttga tgatggccat gttatccatt gttggtaccg ttttgctatt tgtgtatgtt
       61 ttcttgtttt ggtttgagtg aagagtgtag tgaagggttg gtttgtattt ataggtgaga
      121 aaggagattt gcatggcaat cacgtgtaag aatgcatgca tgcatgagtg tgtgtggaga
      181 gagagagatg ctcttacgtt gttgtcggga ttatgtgttg gaaaagagtg acaagtgacg
      241 aacaaaacaa taccactcat catagctccg cttatgcatg ctatgtgtga tttgtagctc
      301 ttgttctgac atgacactat gaacaatttt ttaaaaagaa ggttaaactt ttatttaaat
      361 aaaaacccta tgttaatttg gtttcaatcg agtaagatga gaagtcaatc ttttgatggt
      421 agctagtgat gttggaaata tagttgtgcc agcttaggtc tcatagttga gaccaagctt
      481 cacttcacta gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
      541 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
      601 gtccatttgt atgtgcatgc caaccacagg gttcccctcg gga
//
