LOCUS       sequence-152381-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a 
            visual screen for Cas9-free plants. Enable production of two gRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Gao X, Chen J, Dai X, Zhang D, Zhao Y
  TITLE     An effective strategy for reliably isolating heritable and Cas9-free
            Arabidopsis mutants generated by CRISPR/Cas9-mediated genome 
            editing.
  JOURNAL   Plant Physiol. 2016 May 15. pii: pp.00663.2016.
  PUBMED    27208253
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2016 May 15. pii: pp.00663.2016."
COMMENT     Sequence Label: pHDE-35S-Cas9-mCherry-UBQ
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      253..505
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
ORIGIN
        1 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
       61 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      121 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      181 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      241 caagtgagag ctcttggact cccatgttgg caaaggcaac caaacaaaca atgaatgatc
      301 cgctcctgca tatggggcgg tttgagtatt tcaactgcca tttgggctga attgaagaca
      361 tgctcctgtc agaaattccg tgatcttact caatattcag taatctcggc caatatccta
      421 aatgtgcgtg gctttatctg tctttgtatt gtttcatcaa ttcatgtaac gtttgctttt
      481 cttatgaatt ttcaaataaa ttatcgtatt gagaccgaat tctcgtcgac gagtcagtaa
      541 taaacggcgt caaagtggtt gcagccggca cacacgagtc gtgtttatca actcaaagca
      601 caaatacttt tcctcaacct aaaaataagg caattagcca aaaacaactt tgcgtgtaaa
      661 caacgctcaa tacacgtgtc attttattat tagctattgc ttcaccgcct tagctttctc
      721 gtgacctagt cgtcctcgtc ttttcttctt cttcttctat aaaacaatac ccaaagagct
      781 cttcttcttc acaattcaga tttcaatttc tcaa
//
