LOCUS       sequence-152400-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, 
            Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann 
            CD, Guler AD
  TITLE     Genetically targeted magnetic control of the nervous system.
  JOURNAL   Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
  PUBMED    26950006
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265."
COMMENT     Sequence Label: pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(128..184)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(224..247)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(842..865)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt
       61 gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca
      121 cggatccggg accggggttt tcttccacgt ctcctgcttg ctttaacaga gagaagttcg
      181 tggcgccgct gccgctagcc actcgggagg agcctccacc ccctttgtca tcgtcgtctt
      241 tatagtcgct cccgccaccc ccgcgtgcgc tttcattatc actgtctccc agggtgtgct
      301 tgtcaaagag atattccgcc aagccagatt cgggcgctcc catcttgcgc aagttggtca
      361 cgtggtcacc caattctttg atggctttca cctgctcatt caggtaatgt gtctcaatga
      421 agtcacacaa atgggggtca tttttgtcag tggccagttt gtgcagttcc agtagtgact
      481 gattcacatt tttttccaaa tgtaatgcac actccattgc attcagcccg ctctcccagt
      541 catcacagtc tggtttcttg atatcctgaa ggaagattcg gccacctcgt tggttctgca
      601 gcttcatcag tttctcagca tgttccctct cctcatgaga ttggtgaaga aagtatttgg
      661 caaagttctt caaagccaca tcatcgcggt caaagtagta agacatggac aggtaaacgt
      721 aggaggcgta gagctccagg ttgatctggc ggttgatggc ggcctctgag tcctggtggt
      781 agttctggcg cacctgcgag gtggacgcgg tcgtcatgac gcgggaggag ccgccgccgc
      841 ctttatcatc atcatcttta taatcggagc cgccgccgcc gcgggcgtcg tgcttgagag
      901 tgagcctttc gaagagatac tcgcccagcc cagcctccgg gccacccagc ctgtggaggt
      961 tggtcaggtg gtcacccatc ttcttgataa gcttcacttc ctcatcta
//
