LOCUS       sequence-152405-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GESTALT V4 integrating barcode with Puro and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   McKenna A, Findlay GM, Gagnon JA, Horwitz MS, Schier AF, Shendure J
  TITLE     Whole organism lineage tracing by combinatorial and cumulative 
            genome editing.
  JOURNAL   Science. 2016 May 26. pii: aaf7907.
  PUBMED    27229144
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 May 26. pii: aaf7907."
COMMENT     Sequence Label: pLJM1-EGFP-BarcodeV4
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             75..308
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(519..539)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gttcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg
       61 acatgaacta ctactgctag agattttcca cactgactaa aagggtctga gggatctcta
      121 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
      181 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
      241 taaccagaga gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgaattag
      301 cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt
      361 cattggtctt aaaggtaccg atgcatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg
      421 gtcgtggggc gggcgtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc
      481 gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg
      541 gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg
      601 gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc
      661 caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg
      721 cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc
      781 ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac
      841 ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac
      901 ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg gccg
//
