LOCUS       sequence-152417-         332 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic Cre-dependent expression of TRPV4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, 
            Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann 
            CD, Guler AD
  TITLE     Genetically targeted magnetic control of the nervous system.
  JOURNAL   Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
  PUBMED    26950006
REFERENCE   2  (bases 1 to 332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265."
COMMENT     Sequence Label: pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA
FEATURES             Location/Qualifiers
     source          1..332
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      98..107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    120..153
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    204..237
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gagaggggga aggcctcccc accagaagtg ccactctcgt ctccaggggg ctcagccaca
       61 tccccaggcg ctgcacgggg gccatcacca ggatctgcca tggtggcggt acctctagca
      121 taacttcgta taaagtatcc tatacgaagt tatttgcctt aacccagaaa ttatcactgt
      181 tattctttag aatggtgcaa agaataactt cgtataatgt atgctatacg aagttatgaa
      241 ttcaaataaa atacgaaatg cacgtaacca cgtgcggacc gagcggccgc aggaacccct
      301 agtgatggag ttggccactc cctctctgcg cg
//
