LOCUS       sequence-152418-         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown of NP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Figueiro-Silva J, Gruart A, Clayton KB, Podlesniy P, Abad MA, Gasull
            X, Delgado-Garcia JM, Trullas R
  TITLE     Neuronal pentraxin 1 negatively regulates excitatory synapse density
            and synaptic plasticity.
  JOURNAL   J Neurosci. 2015 Apr 8;35(14):5504-21. doi: 
            10.1523/JNEUROSCI.2548-14.2015.
  PUBMED    25855168
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2548-14"; journalName: "J Neurosci"; date: 
            "2015-04-8- 8"; volume: "35"; issue: "14"; pages: "5504-21"
COMMENT     Sequence Label: pLVTH-shNP1
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    196..229
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(315..529)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(375..394)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    558..576
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
       61 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
      121 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
      181 caagcaaaaa gcagcataac ttcgtataat gtatgctata cgaagttatt cccatgcgac
      241 ggtatcgata agctaaaaag tacagccgcc tcaattcttc tcttgaaaga attgaggcgg
      301 ctgtacgggg atctgtggtc tcatacagaa cttataagat tcccaaatcc aaagacattt
      361 cacgtttatg gtgatttccc agaacacata gcgacatgca aatattgcag ggcgccactc
      421 ccctgtccct cacagccatc ttcctgccag ggcgcacgcg cgctgggtgt tcccgcctag
      481 tgacactggg cccgcgattc cttggagcgg gttgatgacg tcagcgttcg aattcctgca
      541 gccccgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
      601 ctctatcact gatagg
//
