LOCUS       sequence-152420-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of hyperactive Tn5 transposase carrying E54K 
            and L372P mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  TITLE     Linnarsson lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psfTn5
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             715..732
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(785..803)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      799..846
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agcgagcctg agcctgcgta gcggccgtat taccctgaaa cagggcaaca ttaccctgaa
       61 cgcggtgctg gccgaagaaa ttaatccgcc gaaaggcgaa accccgctga aatggctgct
      121 gctgaccagc gagccggtgg aaagtctggc ccaagcgctg cgtgtgattg atatttatac
      181 ccatcgttgg cgcattgaag aatttcacaa agcgtggaaa acgggtgcgg gtgcggaacg
      241 tcagcgtatg gaagaaccgg ataacctgga acgtatggtg agcattctga gctttgtggc
      301 ggtgcgtctg ctgcaactgc gtgaatcttt tactccgccg caagcactgc gtgcgcaggg
      361 cctgctgaaa gaagcggaac acgttgaaag ccagagcgcg gaaaccgtgc tgaccccgga
      421 tgaatgccaa ctgctgggct atctggataa aggcaaacgc aaacgcaaag aaaaagcggg
      481 cagcctgcaa tgggcgtata tggcgattgc gcgtctgggc ggctttatgg atagcaaacg
      541 taccggcatt gcgagctggg gtgcgctgtg ggaaggttgg gaagcgctgc aaagcaaact
      601 ggatggcttt ctggccgcga aagacctgat ggcgcagggc attaaaatct gacagtaaag
      661 gtggatacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac
      721 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      781 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      841 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
      901 cattaagcgc ggcg
//
