LOCUS       sequence-152437-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of double-cysteine variant of Malonyl-coenzyme A 
            synthetase, RpMatB, as scaffold for ATP biosensor - weak binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Vancraenenbroeck R, Kunzelmann S, Webb MR
  TITLE     Development of a range of fluorescent reagentless biosensors for 
            ATP, based on malonyl-coenzyme A synthetase.
  JOURNAL   PLoS One. 2017 Jun 21;12(6):e0179547. doi: 
            10.1371/journal.pone.0179547. eCollection 2017.
  PUBMED    28636641
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-06-21"; volume: "12(6):e0179547. doi"; pages: " 
            10.1371/journal.pone.0179547. eCollection 2017"
COMMENT     Sequence Label: pTEV5_ RpMatB_4
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             65..82
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             104..124
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat atatgtcgta
       61 ctaccatcac catcaccatc acgattacga tatcccaact agtgaaaacc tgtattttca
      121 gggcgctagc atgaacgcca acctgttcgc ccgcctgttc gataagctcg acgaccccca
      181 caagctcgcg atcgaaaccg cggccgggga caagatcagc tacgccgagc tggtggcgcg
      241 ggcgggccgc gtcgccaacg tgctggtggc acgcggcctg caggtcggcg accgcgttgc
      301 ggcgcaaacc gagaagtcgg tggaagcgct ggtgctgtat ctcgccacgg tgcgggccgg
      361 cggcgtgtat ctgccgctca acaccgccta tacgctgcac gagctcgatt acttcatcac
      421 cgatgccgag ccgaagatcg tggtggccga tccgtccaag cgcgacggga tcgcggcgat
      481 tgccgccaag gtcggcgcca cggtggagac gcttggcccc gacggtcggg gctcgctcac
      541 cgatgcggca gctggagcca gcgaggcgtt cgccacgatc gaccgcggcg ccgatgatct
      601 ggcggcgatc ctctacacct cagggacgac cggccgctcc aagggcgcga tgctcagcca
      661 cgacaatttg gcgtcgaact cgctgacgct ggtcgattac tggcgcttca cgccggatga
      721 cgtgctgatc cacgcgctgc cgatctatca cacccatgga ttgttcgtgg ccagcaacgt
      781 cacgctgttc gcgcgcggat cgatgatctt cctgccgaag ttcgatcccg acaagatcct
      841 cgacctgatg gcgcgcgcca ccgtgctgat gggtgtgccg acgttctaca cgcggctctt
      901 gcagagcccg cggctgacca aggagacgac gggccac
//
