LOCUS       sequence-152438-        1590 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of double-cysteine variant of Malonyl-coenzyme A 
            synthetase, RpMatB, as scaffold for ATP biosensor - very weak 
            binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Vancraenenbroeck R, Kunzelmann S, Webb MR
  TITLE     Development of a range of fluorescent reagentless biosensors for 
            ATP, based on malonyl-coenzyme A synthetase.
  JOURNAL   PLoS One. 2017 Jun 21;12(6):e0179547. doi: 
            10.1371/journal.pone.0179547. eCollection 2017.
  PUBMED    28636641
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-06-21"; volume: "12(6):e0179547. doi"; pages: " 
            10.1371/journal.pone.0179547. eCollection 2017"
COMMENT     Sequence Label: pTEV5_ RpMatB_3
FEATURES             Location/Qualifiers
     source          1..1590
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgata tcccaactag tgaaaacctg
       61 tattttcagg gcgctagcat gaacgccaac ctgttcgccc gcctgttcga taagctcgac
      121 gacccccaca agctcgcgat cgaaaccgcg gccggggaca agatcagcta cgccgagctg
      181 gtggcgcggg cgggccgcgt cgccaacgtg ctggtggcac gcggcctgca ggtcggcgac
      241 cgcgttgcgg cgcaaaccga gaagtcggtg gaagcgctgg tgctgtatct cgccacggtg
      301 cgggccggcg gcgtgtatct gccgctcaac accgcctata cgctgcacga gctcgattac
      361 ttcatcaccg atgccgagcc gaagatcgtg gtggccgatc cgtccaagcg cgacgggatc
      421 gcggcgattg ccgccaaggt cggcgccacg gtggagacgc ttggccccga cggtcggggc
      481 tcgctcaccg atgcggcagc tggagccagc gaggcgttcg ccacgatcga ccgcggcgcc
      541 gatgatctgg cggcgatcct ctacacctca gggacggccg gccgctccaa gggcgcgatg
      601 ctcagccacg acaatttggc gtcgaactcg ctgacgctgg tcgattactg gcgcttcacg
      661 ccggatgacg tgctgatcca cgcgctgccg atctatcaca cccatggatt gttcgtggcc
      721 agcaacgtca cgctgttcgc gcgcggatcg atgatcttcc tgccgaagtt cgatcccgac
      781 aagatcctcg acctgatggc gcgcgccacc gtgctgatgg gtgtgccgac gttctacacg
      841 cggctcttgc agagcccgcg gctgaccaag gagacgacgg gccacatgag gctgttcatc
      901 tccgggtcgg cgccgctgct cgccgatacg cattgcgaat ggtcggcgaa gaccggtcac
      961 gccgtgctcg agcgctacgg catgaccgag accaacatga acacctcgaa cccgtatgac
     1021 ggcgaccgcg tccccggcgc ggtcggcccg gcgctgcccg gcgtttcggc gcgcgtgacc
     1081 gatccggaaa ccggcaagga actgccgcgc ggcgacatcg ggatgatcga ggtgaagggc
     1141 ccgaacgtgt tcaagggcta ctggcggatg ccggagaaga ccaagtctga attccgcgac
     1201 gacggcttct tcatcaccgg cgacctcggc aagatcgacg agcgcggcta cgtccacatc
     1261 ctcggccgcg gcaaggatct ggtgatcacc ggcggcttca acgtctatcc gaaggaaatc
     1321 gagagcgaga tcgacgccat gccgggcgtg gtcgaatccg cggtgatcgg cgtgccgcac
     1381 gccgatttcg gcgagggcgt cactgccgtg gtggtgcgcg acaagggtgc cacgatcgac
     1441 gaagcgtgcg tgctgcacgg cctcgacggt cagctcgcca agttcaagat gccgaagaaa
     1501 gtgatcttcg tcgacgacct gccgcgcaac accatgggtg cggtccagaa gaacgtcctg
     1561 cgcgagacct acaaggacat ctacaagtaa
//
