LOCUS       sequence-152450-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transposon-propagation plasmid for Mu-BsaI modified transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Nadler DC, Morgan SA, Flamholz A, Kortright KE, Savage DF
  TITLE     Rapid construction of metabolite biosensors using domain-insertion 
            profiling.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12266. doi: 10.1038/ncomms12266.
  PUBMED    27470466
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12266. doi"; pages: " 
            10.1038/ncomms12266"
COMMENT     Sequence Label: pUCKanR-Mu-BsaI
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(93..115)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
       61 ttttctaagc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggtgacg
      121 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
      181 ggtgttggcg ggtgtcgggg ctggcttaat taaccgctaa gcttgacgcg gagaccgaaa
      241 aacgcgaaag cgtttcacga taaatgcgaa aacggatcga tcctatcgtc aattattacc
      301 tccacgggga gagcctgagc aaactggcct caggcatttg agaagcacac ggtcacactg
      361 cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc
      421 tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat
      481 tatcacttat tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa
      541 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
      601 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
      661 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
      721 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgacacg aaaaacatat
      781 tctcaataaa ccctttaggg aaataggcca ggttt
//
