LOCUS       sequence-152452-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transposon-propagation plasmid for Mu-BsaI modified transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Nadler DC, Morgan SA, Flamholz A, Kortright KE, Savage DF
  TITLE     Rapid construction of metabolite biosensors using domain-insertion 
            profiling.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12266. doi: 10.1038/ncomms12266.
  PUBMED    27470466
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12266. doi"; pages: " 
            10.1038/ncomms12266"
COMMENT     Sequence Label: pUCKanR-Mu-BsaI
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(37..139)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(332..354)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    343..359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(367..373,374..391,392..397))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    412..433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(550..567)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgacaac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
      121 acctcttacg tgccgatcaa catctcattt tcgccaaaag ttggcccagg gcttcccggt
      181 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta
      241 ttcggtcgaa aaggatcgat ccgttttcgc atttatcgtg aaacgctttc gcgtttttcg
      301 gtctccgatg cagatctgga catagctgtt tcctgtgtga aattgttatc cgctcacaat
      361 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      421 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      481 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      541 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      601 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      661 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      721 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
//
