LOCUS       sequence-152455-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Staging plasmid for use in DIP transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Nadler DC, Morgan SA, Flamholz A, Kortright KE, Savage DF
  TITLE     Rapid construction of metabolite biosensors using domain-insertion 
            profiling.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12266. doi: 10.1038/ncomms12266.
  PUBMED    27470466
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12266. doi"; pages: " 
            10.1038/ncomms12266"
COMMENT     Sequence Label: pATT-Dest
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    37..93
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(94..111)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(94..110)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(103..125)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(319..338)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(500..518)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      514..561
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        667..771
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac
       61 tctagaggat ccccgggtac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga
      121 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      181 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      241 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      301 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
      361 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
      421 acaagctggt ctctagcgag agacggttaa gatccggctg ctaacaaagc ccgaaaggaa
      481 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      541 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg
      601 tgtggtcgcc atgatcgcgt agtcaaggtt tcttagacgt caggtggcac ttttcgggga
      661 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      721 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      781 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      841 cacccagaaa cgctggtgaa agtaa
//
