LOCUS       sequence-152458-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid - BsmBI entry sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Nadler DC, Morgan SA, Flamholz A, Kortright KE, Savage DF
  TITLE     Rapid construction of metabolite biosensors using domain-insertion 
            profiling.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12266. doi: 10.1038/ncomms12266.
  PUBMED    27470466
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12266. doi"; pages: " 
            10.1038/ncomms12266"
COMMENT     Sequence Label: pTKEI-Dest
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    109..125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(133..139,140..156,157..162))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        207..284
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(304..323)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
       61 ccagctgcat taatcgtctc tggacatggt atatctcctt gtgtgaaatt gttatccgct
      121 cacaattcca cacattatac gagccggatg attaattgtc aaatttgcgg gatcggagtc
      181 gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta
      241 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
      301 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
      361 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
      421 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
      481 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
      541 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
      601 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
      661 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
      721 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
      781 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
      841 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aa
//
