LOCUS       sequence-152465-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit of (human codon optimized) inactivated Cas9 
            fused to the EDLL Transcriptional Activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pEGB 35s:dCas:EDLL:tNos (GB1190)
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..117
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcatttcatt tggagaggac tccggtattt ttacaacaat taccacaaca aaacaaacaa
       61 caaacaacat tacaatttac tattctagtc gaaatgccca agaagaagag gaaggtggac
      121 aagaagtact ccattgggct cgctatcggc acaaacagcg tcggctgggc cgtcattacg
      181 gacgagtaca aggtgccgag caaaaaattc aaagttctgg gcaataccga tcgccacagc
      241 ataaagaaga acctcattgg cgccctcctg ttcgactccg gggaaacggc cgaagccacg
      301 cggctcaaaa gaacagcacg gcgcagatat acccgcagaa agaatcggat ctgctacctg
      361 caggagatct ttagtaatga gatggctaag gtggatgact ctttcttcca taggctggag
      421 gagtcctttt tggtggagga ggataaaaag cacgagcgcc acccaatctt tggcaatatc
      481 gtggacgagg tggcgtacca tgaaaagtac ccaaccatat atcatctgag gaagaagctt
      541 gtagacagta ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc
      601 aaatttcggg gacacttcct catcgagggg gacctgaacc cagacaacag cgatgtcgac
      661 aaactcttta tccaactggt tcagacttac aatcagcttt tcgaagagaa cccgatcaac
      721 gcatccggag ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc ccggcggctc
      781 gaaaacctca tcgcacagct ccctggggag aagaagaacg gcctgtttgg taatcttatc
      841 gccctgtcac tcgggctgac ccccaacttt aaatct
//
