LOCUS       sequence-152467-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 549, Emission: 
            565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(138..848)
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     regulatory      845..854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agctgcataa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
       61 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
      121 tctagagtcg cggccgctta cttgtacagc tcgtccatgc cgccggtgga gtggcggccc
      181 tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac
      241 ttgatgtcga cgatgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc
      301 ttgacctcgg aggtgtagtg gccgccgtcc ttcagcttca gcctcatctt gatcttgccc
      361 ttcagggcac cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc
      421 ttctgcatca cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg
      481 aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgtag
      541 ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttgaagta gtcggggatg
      601 tcggcggggt gcttcacgta ggccttggag ccgtaggtga aatgagggga caggatgtcc
      661 caggcgaagg gcagggggcc acccttggtc accttcagct tagcggtctg aaagccctcg
      721 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc
      781 atgcgcacct tgaagcgcat gaactccttg atgatggcca tgttattctc ctcgcccttg
      841 ctcaccatgg tggcgaccgg tggatcccct tcaaggtcat cgtgatgctg agaagtttcg
      901 ttgataacct gtccatctct agtttcaacc
//
