LOCUS       sequence-152471-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit of (human codon optimized) inactivated Cas9 
            fused to the VP64 Transcriptional Activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pEGB 35s:dCas:VP64:tNos (GB1189)
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             89..109
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttggagagg actccggtat ttttacaaca attaccacaa caaaacaaac aacaaacaac
       61 attacaattt actattctag tcgaaatgcc caagaagaag aggaaggtgg acaagaagta
      121 ctccattggg ctcgctatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta
      181 caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa
      241 gaacctcatt ggcgccctcc tgttcgactc cggggaaacg gccgaagcca cgcggctcaa
      301 aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat
      361 ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt
      421 tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga
      481 ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag
      541 tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg
      601 gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt
      661 tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg
      721 agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct
      781 catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc
      841 actcgggctg acccccaact ttaaatctaa c
//
