LOCUS       sequence-152472-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit of (human codon optimized) inactivated Cas9 
            fused to the VP64 Transcriptional Activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pEGB 35s:dCas:VP64:tNos (GB1189)
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(15..267)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(294..443)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(444..464)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(468..488)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(492..512)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcttggcaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
       61 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
      121 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
      181 attcaacaga aattatatga taatcgtcgc aagaccggca acaggattca atcttaagaa
      241 actttattgc caaatgtttg aacgatcggg atcgaagatc cattccaagc tcataacatg
      301 tccagatcaa agtcgtccaa agcgtcggag cccaacatgt ccaagtcgaa gtcatctagc
      361 gcgtcagaac ccaacatgtc gaggtcaaag tcgtccaaag catctgaccc aagcatgtct
      421 aagtcgaagt catccaatgc gtctaccttt ctcttcttct taggatctac ctttctcttc
      481 ttcttgggat cgaccttacg cttctttttt gggtccgaat cagccctgct gtctccaccg
      541 agctgagaga ggtcgattct tgtttcatag agccccgtaa ttgactgatg aatcagtgtg
      601 gcgtccagga cctcctttgt agaggtgtac cgctttctgt ctatggtggt gtcgaagtac
      661 ttgaaggctg caggcgcgcc caagttggtc agagtaaaca agtggataat gttttctgcc
      721 tgctccctga tgggcttatc cctgtgctta ttgtaagcag aaagcacctt atcgaggtta
      781 gcgtcggcga ggatcactct tttggagaat tcgcttattt gctcgatgat ctcatcaagg
      841 tagtgtttgt gttgttccac gaacagctgc ttctgctcat tatcttcggg agatcctttg
      901 agcttttcat agtggctggc cagatacaag aaattaacgt atttagaggg cagtgccagc
      961 tcgttacctt tctgcag
//
