LOCUS       sequence-152473-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA shuttle vector; module 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE5
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..152)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(427..444)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(427..443)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(436..458)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(652..671)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     771..793
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(831..849)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        917..1021
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      121 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca gaagacttca atcactactt
      181 cgactctagc tgtatataaa ctcagcttcg ttttcttatc taagcgatgt gggacttttg
      241 aagattgttt tcaacttagt accgttgata gatacctctc cccgaaactc gggaatatcg
      301 ccacaaatag cttcccagcg ttttctggcc tctgtgcaag tttaattgct gcagcagctg
      361 ctgcaccgga tgatattcgc aatgagacca atctagatgc attcgcgagg taccgagctc
      421 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      481 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      541 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
      601 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
      661 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
      721 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      781 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
      841 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      901 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      961 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1021 tatgagtat
//
