LOCUS       sequence-152474-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 558, Emission: 583.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DsRed2-Nucleus-4
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             53..727
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(233..253)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     645..668
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             743..763
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             767..787
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             791..811
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcagagctg gtttagtgac cgtcagatcc gctagcgcta ccggtcgcca ccatggcctc
       61 ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg cgcatggagg gcaccgtgaa
      121 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gccacaacac
      181 cgtgaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
      241 ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc ccgactacaa
      301 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      361 cgtggcgacc gtgacccagg actcctccct gcaggacggc tgcttcatct acaaggtgaa
      421 gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg cagaagaaga ccatgggctg
      481 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agacccacaa
      541 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcca tctacatggc
      601 caagaagccc gtgcagctgc ccggctacta ctacgtggac gccaagctgg acatcacctc
      661 ccacaacgag gactacacca tcgtggagca gtacgagcgc accgagggcc gccaccacct
      721 gttcctgaga tctcgagctg atccaaaaaa gaagagaaag gtagatccaa aaaagaagag
      781 aaaggtagat ccaaaaaaga agagaaaggt aggatccacc ggatctagat aactgatcat
      841 aatcagccat accacatttg tagaggtttt acttgcttta aa
//
