LOCUS       sequence-152479-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit of (human codon optimized) inactivated Cas9 
            fused to the BRD Transcriptional Repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pEGB 35s:dCas:BRD:tNos (GB1172)
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             96..116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catttcattt ggagaggact ccggtatttt tacaacaatt accacaacaa aacaaacaac
       61 aaacaacatt acaatttact attctagtcg aaatgcccaa gaagaagagg aaggtggaca
      121 agaagtactc cattgggctc gctatcggca caaacagcgt cggctgggcc gtcattacgg
      181 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
      241 taaagaagaa cctcattggc gccctcctgt tcgactccgg ggaaacggcc gaagccacgc
      301 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
      361 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
      421 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
      481 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg
      541 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca
      601 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca
      661 aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaacg
      721 catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg
      781 aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg
      841 ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca
      901 agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg gcccagatcg
      961 gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc attctgc
//
