LOCUS       sequence-152480-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit of (human codon optimized) inactivated Cas9 
            fused to the BRD Transcriptional Repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pEGB 35s:dCas:BRD:tNos (GB1172)
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(13..265)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(337..357)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttggcaattc ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
       61 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
      121 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
      181 tcaacagaaa ttatatgata atcgtcgcaa gaccggcaac aggattcaat cttaagaaac
      241 tttattgcca aatgtttgaa cgatcgggat cgaagatcca ttccaagcct aacattccat
      301 attaacacca aaaagtctaa gagtcttaga attacccacc ttcctcttct tcttgggcga
      361 atcagccctg ctgtctccac cgagctgaga gaggtcgatt cttgtttcat agagccccgt
      421 aattgactga tgaatcagtg tggcgtccag gacctccttt gtagaggtgt accgctttct
      481 gtctatggtg gtgtcgaagt acttgaaggc tgcaggcgcg cccaagttgg tcagagtaaa
      541 caagtggata atgttttctg cctgctccct gatgggctta tccctgtgct tattgtaagc
      601 agaaagcacc ttatcgaggt tagcgtcggc gaggatcact cttttggaga attcgcttat
      661 ttgctcgatg atctcatcaa ggtagtgttt gtgttgttcc acgaacagct gcttctgctc
      721 attatcttcg ggagatcctt tgagcttttc atagtggctg gccagataca agaaattaac
      781 gtatttagag ggcagtgcca gctcgttacc tttctgcagc tcgcccgcac tagcgagcat
      841 tcgtttccgg ccgttttcaa gctcaaagag agagtacttg ggaagcttaa tgatgaggtc
      901 ttttttgacc tctttatatc ctttcgcctc gagaaagtcg atggggtttt tttcgaagct
      961 tgatcgctcc atgattgtga tgcccagcag ttccttgacg cttttgag
//
