LOCUS       sequence-152481-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB076i5
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(459..482)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atcaatattg ttgatgataa tattaagaag taccacacgg aatataccta aacggagcca
       61 atgtacgcaa aaaaccccgc ttcagcgggg ttttttcgcc aaaaaaaacc ccgccctgtc
      121 aggggcgggg ttttttttta gggccctact gactgttagc gtttcggcgc cggataatgc
      181 agcgtggtga tcagaccatc cgcaaccgtg ctatggtgat gcaccagttc tgccagggta
      241 ttgaagcgag attcactaga aacgtacagt ttgccatcgc tggcggtgtt gatacggtag
      301 tgatacacgc gaccttcata acgcagagaa atgctgcgct ggcccgggct actttcactt
      361 tcacgaacca gaaaagaacc attaatgcca gagctcagca ggtattcggc cgcgttacgg
      421 ctcacagggc catgatacca gctgtgtttt tccagactct tgtcatcgtc gtccttgtag
      481 tctacgaatt ttaccctcgc ttccacgaca acaccgataa tcttgcagtt tccgttgata
      541 ggagtcatag gccatgaagg attcaggcct ttcaggtact tctgaccgcc atctatgacc
      601 agtttcttga atgttgcttc gttcgcgtca gtcagtttgg ctacaacaag gcttccattc
      661 actggctccc gtccagtatc tactaacacc atatgacctt cagggatgct ttgacctaca
      721 ggtgaggtca tggaatcacc ttcaaccttc agccagaatc cattg
//
