LOCUS       sequence-152486-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for (human codon optimized) Cas9 plant 
            expression driven by the 35S promoter. Specially conceived to be 
            linked with the TU of the kanamycin resistance gene GB1181..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttcatttgg agaggactcc ggtattttta caacaattac cacaacaaaa caaacaacaa
       61 acaacattac aatttactat tctagtcgaa atggacaaga agtactccat tgggctcgat
      121 atcggcacaa acagcgtcgg ctgggccgtc attacggacg agtacaaggt gccgagcaaa
      181 aaattcaaag ttctgggcaa taccgatcgc cacagcataa agaagaacct cattggcgcc
      241 ctcctgttcg actccgggga aacggccgaa gccacgcggc tcaaaagaac agcacggcgc
      301 agatataccc gcagaaagaa tcggatctgc tacctgcagg agatctttag taatgagatg
      361 gctaaggtgg atgactcttt cttccatagg ctggaggagt cctttttggt ggaggaggat
      421 aaaaagcacg agcgccaccc aatctttggc aatatcgtgg acgaggtggc gtaccatgaa
      481 aagtacccaa ccatatatca tctgaggaag aagcttgtag acagtactga taaggctgac
      541 ttgcggttga tctatctcgc gctggcgcat atgatcaaat ttcggggaca cttcctcatc
      601 gagggggacc tgaacccaga caacagcgat gtcgacaaac tctttatcca actggttcag
      661 acttacaatc agcttttcga agagaacccg atcaacgcat ccggagttga cgccaaagca
      721 atcctgagcg ctaggctgtc caaatcccgg cggctcgaaa acctcatcgc acagctccct
      781 ggggagaaga agaacggcct gtttggtaat cttatcgccc tgtcactcgg gctgaccccc
      841 aactttaaat ctaacttcga cctggccgaa gatgccaagc ttcaactgag caaagacacc
      901 tacgatgatg atctcgacaa tctgct
//
