LOCUS       sequence-152488-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for kanamycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pEGB3omega1R Tnos:NptII:Pnos-SF (GB1181)
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(62..241)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    503..527
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ctgcgtgcaa tccatcttgt tcaatcatgc ctcgatcgag ttgagagtga atatgagact
       61 ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat
      121 ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat
      181 gtgcttagct cattaaactc cagaaacccg cggctcagtg gctccttcaa cgttgcggtt
      241 ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataccgtgac
      301 tcccttaatt ctcatgtatc tcctgacagc gaaatgattg atgaagaaca atggtggatg
      361 aagaacaaag aaggagggag cttttgttca agatgaacaa agaacaatag tggatgaaga
      421 acaaagtgaa aaaaataaaa aaaaatgtat ggttaaataa agagtaaagt taccattgag
      481 actccgtcat gagaccgaat tctgacagga tatattggcg ggtaaaccta agagaaaaga
      541 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
      601 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
      661 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcatct gaaaacgaca
      721 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
      781 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
      841 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
      901 aggacttg
//
