LOCUS       sequence-152489-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for kanamycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pEGB3omega1R Tnos:NptII:Pnos-SF (GB1181)
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      217..411
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct
       61 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc
      121 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct
      181 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta
      241 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata
      301 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg
      361 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagagt
      421 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag
      481 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca
      541 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg
      601 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc
      661 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa
      721 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc
      781 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctc
//
