LOCUS       sequence-152490-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the human codon optimized CDS of Cas9 protein with mutated 
            (D10A, H840A) and inactivated catalytic domains as a level 0 
            GoldenBraid part for C-terminal fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pdCas9 (GB1079)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(100..111)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gattatcgaa cttccgttgt gtgatcagtt tggcgttcag cagctgccgc caataatttt
       61 tcattttctt gacaacttct tctgagggga cgttatcact cttccctcta tttttatcgg
      121 atcttgtcaa cactttatta tcaatagaat catctttgag aaaagactgg ggcacgatgg
      181 catccacgtc gtagtcggag agccgattga tgtccagttc ctgatccacg tacatgtccc
      241 tgccgttctg caggtagtac aggtagagct tctcattctg aagctgggtg ttttcaactg
      301 ggtgttcctt aaggatttgg gaccccagtt cttttatacc ctcttcaatc ctcttcatcc
      361 tttccctact gttcttctgt cccttctggg tagtttggtt ctctcgggcc atctcgataa
      421 cgatattctc gggcttatgc cttcccatta ctttgacgag ttcatccacg accttaacgg
      481 tctgcagtat tccctttttg atagctgggc tacctgcaag attagcgatg tgctcgtgga
      541 gactgtcccc ctggccagaa acttgtgctt tctggatgtc ctccttaaag gtgagagagt
      601 catcatggat caactgcatg aagttccggt tggcaaatcc atcggactta agaaaatcca
      661 ggattgtctt tccactctgc ttgtctcgga tcccattgat cagttttctt gacagccgcc
      721 cccatcctgt atatcggcgc ctcttgagct gtttcatgac tttgtcgtcg aagagatgag
      781 cgtaagtttt caagcgttct tcaatcatct ccctatcttc aaacaacgta agggtgagga
      841 caatgtcctc aagaatgtcc tcgttctcct cattgtccag
//
