LOCUS       sequence-152494-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pHAGE lentiviral vector with dsRed driven by the human E-cadherin 
            promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, 
            comprises the Z-cad dual sensor. Use 2nd generation packaging 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Toneff MJ, Sreekumar A, Tinnirello A, Hollander PD, Habib S, Li S, 
            Ellis MJ, Xin L, Mani SA, Rosen JM
  TITLE     The Z-cad dual fluorescent sensor detects dynamic changes between 
            the epithelial and mesenchymal cellular states.
  JOURNAL   BMC Biol. 2016 Jun 17;14(1):47.
  PUBMED    27317311
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-06-17"; volume: "14(1)"; pages: "47"
COMMENT     Sequence Label: pHAGE-E-cadherin-RFP
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..709)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(94..117)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(292..311)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     509..529
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     561..579
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      706..715
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttgtaa tccagaggtt gattaggatc cctacaggaa caggtggtgg cggccctcgg
       61 cgcgctcgta ctgctccacg atggtgtagt cctcgttgtg ggaggtgatg tccagcttgg
      121 agtccacgta gtagtagccg ggcagctgca cgggcttctt ggccatgtag atggacttga
      181 actccaccag gtagtggccg ccgtccttca gcttcagggc cttgtggatc tcgcccttca
      241 gcacgccgtc gcgggggtac aggcgctcgg tggaggcctc ccagcccata gtcttcttct
      301 gcattacggg gccgtcggag gggaagttca cgccgatgaa cttcaccttg tagatgaagg
      361 agccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca
      421 tcacgcgctc ccacttgaag ccctcgggga aggacagctt cttgtagtcg gggatgtcgg
      481 cggggtgctt cacgtacacc ttggagccgt actggaactg gggggacagg atgtcccagg
      541 cgaagggcag ggggccgccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg
      601 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgc
      661 gcaccttgaa gcgcatgaac tccttgatga cgtcctcgga ggaggccatg gcggccgcgg
      721 ctggccgggg acgccgagcg agggcaggcg ccgggtgcgg tcgggtcggg ccgggctgga
      781 gcgggctgga gtctgaactg acttccgcaa gctcacaggt gctttgcagt tccgacgcca
      841 ctgagagggg gtgcgtggct gcagccaggt gagccccgga ggcaccgccc cccgtaccgc
      901 tgattggctg a
//
