LOCUS       sequence-152496-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FUGW lentiviral vector with constiutively expressed destabilized GFP
            (d2GFP) regulated by 5 miR-200 family binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Toneff MJ, Sreekumar A, Tinnirello A, Hollander PD, Habib S, Li S, 
            Ellis MJ, Xin L, Mani SA, Rosen JM
  TITLE     The Z-cad dual fluorescent sensor detects dynamic changes between 
            the epithelial and mesenchymal cellular states.
  JOURNAL   BMC Biol. 2016 Jun 17;14(1):47.
  PUBMED    27317311
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-06-17"; volume: "14(1)"; pages: "47"
COMMENT     Sequence Label: FUGW-d2GFP-200
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(48..89)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(483..504)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     832..851
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattggc catttgttcc
       61 atgtgagtgc tagtaacagg ccttgtgtcc tgctagctaa tactggacgt aatgccgtaa
      121 gttctctaag ttctcttaat actggccgta atgatggaaa gttctctaag ttctcttaat
      181 actggacgta atgatgaaag ttctctaagt tctcttaaca ctggacgtaa agatggaagt
      241 tctctaagtt ctcttaacac tggacgtaac gatgtcctag ctacagcctt cagcaagcct
      301 ccagaattcg cctacacatt gatcctagca gaagcacagg ctgcagggtg acggtccatc
      361 ccgctctcct gggcacaaga catgggcagc gtgccatcat cctgctcctc cacctccggc
      421 gggaagccat ggctaagctt cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
      481 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
      541 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
      601 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
      661 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
      721 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
      781 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
      841 ttgccgtcgt ccttgaa
//
