LOCUS       sequence-152499-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB106
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(153..277)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(299..644)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(336..356)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggcccc tcgaggggcc gccatagtga ctggatatgt tgtgttttac
      181 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
      241 tttacgtttc tcgttcagct tttttgtaca aacttgttga taactctaga gtcccccgtg
      301 ttctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg
      361 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
      421 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
      481 cactgtcggc agaggcatct tcaacgatgg cctttccttt atcgcaatga tggcatttgt
      541 aggagccacc ttccttttcc actatcttca caataaagtg acagatagct gggcaatgga
      601 atccgaggag gtttccggat attacccttt gttgaaaagt ctcaattgcc ctttggtctt
      661 ctgagactgt atctttgata tttttggagt agacaagtgt gtcgtgctcc accatgttga
      721 cgaagatttt
//
