LOCUS       sequence-152502-         592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB109
FEATURES             Location/Qualifiers
     source          1..592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(112..236)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             247..312
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      331..583
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
       61 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
      121 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
      181 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgat
      241 aacagcgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
      301 gacgatgaca agtagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
      361 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
      421 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
      481 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
      541 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt gg
//
