LOCUS       sequence-152507-       10312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FUGW lentiviral vector with constiutively expressed destabilized GFP
            (d2GFP) regulated by 5 miR-200 family binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Toneff MJ, Sreekumar A, Tinnirello A, Hollander PD, Habib S, Li S, 
            Ellis MJ, Xin L, Mani SA, Rosen JM
  TITLE     The Z-cad dual fluorescent sensor detects dynamic changes between 
            the epithelial and mesenchymal cellular states.
  JOURNAL   BMC Biol. 2016 Jun 17;14(1):47.
  PUBMED    27317311
REFERENCE   2  (bases 1 to 10312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-06-17"; volume: "14(1)"; pages: "47"
COMMENT     Sequence Label: FUGW-d2GFP-200
FEATURES             Location/Qualifiers
     source          1..10312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             815..995
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1042..1167
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1660..1893
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2078..2122
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2420..2537
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3815
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3652..3671
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3854..3856,3857..3859,3860..4570,4571..4576,
                     4577..4699)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(3899..3920)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4160..4179)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4507..4528
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           4922..4963
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     misc_feature    4987..5576
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5040..5060)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5458..5469)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6117..6297
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6323..6340)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6329..6553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6599..7027
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6686..6705)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6896..6917
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7036..7056)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7041..7370
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7221..7356
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7418..7465
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7484..7858
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7988..8109
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8025..8044)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8079..8098
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8158..8174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8158..8174)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8171..8193)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8182..8198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8206..8212,8213..8230,8231..8236))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8251..8272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8389..8406)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8560..9148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8640..8659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9319..10110,10111..10179))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9942..9961
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10180..10284)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctctg tactgggtct ctctggttag accagatctg
      841 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      901 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      961 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     1021 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     1081 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     1141 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     1201 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     1261 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1321 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1381 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1441 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1501 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1561 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1621 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1681 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1741 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1801 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1861 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1921 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1981 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     2041 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     2101 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     2161 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     2221 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     2281 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2341 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
     2401 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2461 aaagaatagt agaaataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2521 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta
     2581 acccgtgtcg gctccagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     2641 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     2701 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     2761 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     2821 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     2881 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     2941 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     3001 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     3061 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
     3121 aactgggggt tggggggagc gcacaaaatg gcggctgttc ccgagtcttg aatggaagac
     3181 gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga
     3241 acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg
     3301 gggcaccatc tggggacccc tgacgtgaag tttgtcactg actggagaaa ctcgggtttg
     3361 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     3421 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     3481 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     3541 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     3601 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     3661 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     3721 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3781 ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttgggctgca ggtcgactct
     3841 agaggatccc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     3901 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     3961 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     4021 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     4081 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4141 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4201 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4261 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     4321 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     4381 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     4441 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     4501 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     4561 gctgtacaag aagcttagcc atggcttccc gccggaggtg gaggagcagg atgatggcac
     4621 gctgcccatg tcttgtgccc aggagagcgg gatggaccgt caccctgcag cctgtgcttc
     4681 tgctaggatc aatgtgtagg cgaattctgg aggcttgctg aaggctgtag ctaggacatc
     4741 gttacgtcca gtgttaagag aacttagaga acttccatct ttacgtccag tgttaagaga
     4801 acttagagaa ctttcatcat tacgtccagt attaagagaa cttagagaac tttccatcat
     4861 tacggccagt attaagagaa cttagagaac ttacggcatt acgtccagta ttagctagca
     4921 ggacacaagg cctgttacta gcactcacat ggaacaaatg gccaattcga tatcaagctt
     4981 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5041 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5101 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5161 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5221 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5281 ctccctattg ccacggcgga actcatcgcc cgcctgcctt gcccgctgct ggacaggggc
     5341 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     5401 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtccttcg
     5461 gccctcaatc caagcggacc ttccttcccg cggcctgctg ccggctctgc gggcctcttc
     5521 cgcgtctttc gccttcgccc tcagacgagt cggatctccc tttgggcgct ccccgcatcg
     5581 atgtcgacct cgagaccggc cgaactcgaa gacctagaaa aaacattgga gcaatcacaa
     5641 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     5701 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     5761 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5821 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     5881 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     5941 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     6001 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     6061 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     6121 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6181 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6241 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     6301 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6361 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6421 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6481 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6541 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6601 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6661 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6721 ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt
     6781 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6841 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6901 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6961 gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7021 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7081 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7141 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7201 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7261 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7321 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     7381 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     7441 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     7501 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     7561 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     7621 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     7681 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     7741 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     7801 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     7861 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7921 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7981 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8041 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8101 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8161 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8221 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8281 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8341 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8401 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8461 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8521 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8581 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8641 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8701 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     8761 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8821 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8881 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8941 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9001 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9061 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9121 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9181 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9241 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9301 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9361 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9421 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9481 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9541 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9601 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9661 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9721 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9781 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9841 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9901 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9961 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10021 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10081 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10141 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10201 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10261 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
