LOCUS       sequence-152511-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB134
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(99..105,106..123,124..129))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(166..290)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
       61 tcctgaaaat ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata
      121 aagtgtaaag cctggggtgc ctaatgcggc ccctcgaggg gccgccatag tgactggata
      181 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
      241 atatttatat cattttacgt ttctcgttca gcttttttgt acaaacttgt tgataactct
      301 agatggtgga acttcgctct cgactgcagg aagatcagac agaactcaac aattcggtgt
      361 cgatctcgga tgggaacgtg gaagtaggca agccaaaagc ttgaagtctg tgcttcactg
      421 atcacggtgc aatgatcgct tgaggaaatc gtctgaacac tgatttgatt gatgggaacc
      481 ccaggccctt gcgtggtact tataggcccc ataatgtgct tccttggctc tcacacaacg
      541 agcgcatgaa atctttctaa attcctcgag acatcgctgg tccatcctag aaccctctcg
      601 atacctcgag gctgtgcagg tgagagcgac atcgaatgct cgaggccatg aacgaggtgt
      661 gtactgtccc ggtgattcta gtaccatctc gtcgtcgaat ttcacggtgg ccgacttctc
      721 tagaagtatg catggccgtg ctccatcacg tcggaattcg gctgatcccg gtgaacccag
      781 gccatcttcc cgggaccgga agtctcataa taatctcgaa
//
